Design and Evaluation and Synthesis a Starch-Capped Silver NanoParticles Sensor and Determination trace Sulfacetamide Drug in the Presence Sodium borohydride in Blood and Urine Samples with Kinetic Spectrophotometric Method

Document Type: Research Paper


A new Kinetic Spectrophotometic method for the Determination of trace amount Sulfacetamide Drug into real samples has been described based with silver nanoparticles starch-capped sensor, by sodium borohydride. The reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 347.5 nm. 1.0×10-2 molL-1 silver nanoparticles starch-capped sensor, 2.0×10-3 mol L-1 sodium borohydride at 25oC, calibration graph in the range of 0.1-6.0 μg L-1 sulfacetamide drug. The absorbance is linear from 0.02 up to 8.0 μg L−1 in aqueous solution with repeatability (RSD) of 0.9% at a concentration of 6.0 μg L−1 and a detection limit of 2.3 μg L−1 by the fixed-time method of 7.0 min. The relative standard deviation for 6.0 μg L-1 sulfacetamide drug is %95. No serious interference was identified. The applicability of the method was demonstrated by the determination of the Sulfacetamide drug in urine and blood samples.