Evaluation of the antioxidant activity of Thymus Using Electrochemical Methods and radical scavenging method

Document Type : Research Paper


1 Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


Thymus is regarded as one of the medicinal species, which according to obtained chromatograms of floral water of Thymus; one of the main components is thymol as strong antioxidant. In this study the appropriate and physically powerful correlation was found between the anti-radical and oxidation potentials. The antioxidant activity of the Thymus was measured by using square wave and cyclic voltammetry methods and 2, 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) method in the concentration range of 1% to 2.5% V/V. Electrochemical methods are used based on the relationship between oxidation power and antioxidant strength, which compared with ascorbic acid as standard. Based on the results of electrochemical methods, these methods have several advantages over the DPPH∙ method such as simplicity, cheaper operating costs, short integration time, high speed, low solvent and sample usage. Moreover, the square wave voltammetry method is more sensitive method for evaluation the antioxidant activities compared to DPPH and cyclic voltammetry methods.