Purification and Characterization of Milk Clotting Enzyme Produced by Rhizomucor Rmiehei

Document Type : Research Paper




Milk clotting enzyme (M CE) produced by: Rhizomucor miehei was purified and characterized.
The enzyme was purified 220.29-fold with specific activity about 14444.2 U/mg protein by
ultrafiltration, ammonium sulfate fractionation, Sephacryl S-300 chromatography. The maximum
enzyme activity was at 65°C.
The milk clotting activity was decreased steadily as pH is increased and indicated maximum
activity at pH 5.3.Ferthermore we did some computational methods for understanding the changes
in energy.