Document Type : Research Paper
A new, simple, rapid, and cheap method for determination of trace amounts of nitrite ion is reported.
Determination is based on reduction of bromate ion by nitrite ion, in the presence of murexide in
acidic media. During reaction of bromate ion with nitrite ion, the reaction mixture is decolorized.
The absorbance changes is then monitored spectrophotometricaly at 520 nm, at 25 °C. Dynamic
range of calibration curve for nitrite ion is in the range of 1-22 ig.mL 1 . The effect of interfering
ions was investigated. This method was successfully used for determinination of nitrite ion
concentration in sausages.