Coating of Iron Oxide Nanoparticles with Human and Bovine Serum Albumins: A Thermodynamic Approach

Document Type : Research Paper




In this research, the Magnetite nanoparticles (Fe304) were prepared by coprecipitation of Fe3+ and
FE solution in alkaline medium. Two kinds of surfactants, cetyl tri methyl ammonium bromide
(CTAB) and cetyl pyridinum chloride (CPC) were used in the synthesis. Fe304 Nanoparticles were
coated with human serum albumin (HSA) and bovine serum albumin (BSA). Characteristics of
coated magnetic nanoparticles and no coated were carried out using scanning electron microscopy,
X-ray diffraction and FT-IR spectroscopy. The interactions of colloidal iron oxide nanoparticles with
serum albumins, including BSA and EISA, were investigated by UV-Vis spectroscopy at process
different pH and temperatures. The thermodynamic parameters of coating were obtained from Van't
Hoof equation. The results revealed that both AM: and ASo of reactants positive values. These results
also demonstrate that p1-I can play an important role on adsorption of proteins on iron oxide